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HOME > J Korean Soc Clin Toxicol > Volume 14(2); 2016 > Article
Genoprotective Effect of Melatonin Against to the Genotoxicity of Glyphosate on Human Blood Lymphocytes
Jung-Gyu Kim, Woo-Ik Choi, Jae-Ho Lee, In-Jang Choi, Sang-Chan Jin
Journal of The Korean Society of Clinical Toxicology 2016;14(2):144-150
DOI: https://doi.org/10.22537/jksct.2016.14.2.144
Published online: December 31, 2016
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1Department of Emergency Medicine, School of Medicine, Keimyung University, Dongsan Medical Center
2Department of Emergency Medicine, School of Medicine, Keimyung University, Dongsan Medical Center
3Department of Anatomy, College of Medicine, Keimyung University
4Department of Anatomy, College of Medicine, Keimyung University
5Department of Emergency Medicine, School of Medicine, Keimyung University, Dongsan Medical Center

Purpose: Glyphosate is a widely used non-selective herbicide. Previous studies have shown that glyphosate has genotoxicity, and that even low-doses of glyphosate can cause DNA damage. Melatonin is a hormone produced and secreted by the pineal gland that is known to be a potent anti-carcinogen, anti-oxidant, and genetic protector. This study was conducted to investigate the genoprotective effect of melatonin against glyphosate in human blood lymphocytes. Methods: Human peripheral blood was obtained from 15 young, healthy volunteers and cultured under four different toxicologic conditions. The four groups consisted of a control group, glyphosate only group (300 ng/mL), glyphosate with low level of melatonin group ($50{mu}M$), and glyphosate with high level of melatonin group ($200{mu}M$). The mean Sister Chromatid Exchange (SCE) frequency of each group was then analyzed. Results: Glyphosate exposed groups had a higher mean SCE frequency ($10.33{pm}2.50$) than the control group ($6.78{pm}2.31$, p<0.001). Interestingly, the group that received a low-level of melatonin had a lower mean SCE frequency ($8.67{pm}2.58$) than the glyphosate-only group, while the group that received a high level of melatonin had a much lower mean SCE frequency ($8.06{pm}2.50$) than the glyphosate-only group. There was statistical significance. Conclusion: Melatonin exerted a potent gene protective effect against the genotoxicity of glyphosate on human blood lymphocytes in a dose-dependent fashion.

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JKSCT : Journal of The Korean Society of Clinical Toxicology